Because conservation efforts are typically species-based, these results will be important for facilitating successful conservation management strategies.Ĭopyright © 2013 Elsevier Inc. The DNA barcoding data support these recommended taxonomic revisions. luteola should not be considered separate subspecies. triunguis should be elevated to species status (Terrapene mexicana), and mexicana and yucatana should be included in this group as subspecies. The DNA barcoding results indicated that all taxa were different species (>2% sequence divergence) except for T. Genetic divergence showed a clear separation between other species (0.02 to 0.12), while their relationship with the olive ridley sea turtle (Lepidochelys olivacea) was. bauri, and Terrapene coahuila were not well resolved for some of the analyses. Sequences of nearly 700 bp of Kemp’s ridley were aligned among them and compared to seven different sea turtle species all new sequences will be added to the BOLD database. luteola lacked distinction phylogenetically, and Terrapene nelsoni was confirmed to be the sister taxon of T. triunguis, and this clade was paraphyletic to the rest of T. are made of metal or plastic and are clipped onto the turtles flipper(s). yucatana formed a monophyletic clade with T. The debate centers on the genetic difference between the green sea turtle and. The sequence data were analyzed using maximum likelihood and Bayesian phylogenetic inference, a molecular clock, AMOVAs, SAMOVAs, haplotype networks, and pairwise percent sequence divergence comparisons. In addition, the mtDNA gene commonly used for DNA barcoding (cytochrome oxidase c subunit I COI) was amplified and sequenced to calculate pairwise percent DNA sequence divergence comparisons for each Terrapene taxon.
DNA was extracted, and the mitochondrial DNA (mtDNA) cytochrome b (Cytb) and nuclear DNA (nucDNA) glyceraldehyde-3-phosphate-dehydrogenase (GAPD) genes were amplified via polymerase chain reaction and sequenced. A study led by University of Florida researchers is the first to sequence environmental DNA, or eDNA, from sea turtles genetic material shed as they travel over beaches and in water. Tissue samples were obtained for all taxa except for Terrapene nelsoni klauberi.
SEATURTLE GENE SEQUENCE S SERIES
( 1987 ) obtained a series of Ychromosome - specific DNA sequences that could be used. by obtaining DNA sequence data from a broad geographic range and from all four recognized species and 11 subspecies within the genus. SEXING THE SEA TURTLE Suzanne Demas Stephen Wachtel l'niversity of.
The goal of this study was to re-assess the classification of Terrapene spp. Previous comprehensive phylogenetic studies focused primarily on morphology. The classification of the American box turtles (Terrapene spp.) has remained enigmatic to systematists.